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CD25 抗体 (FITC)

Name: CD25 抗体 (FITC)
SKU: ABIN6655112
Availability: OutOfStock

IL2RA 适用: 人 FACS, IHC 宿主: 小鼠 Monoclonal BC96 FITC

Flow Cytometry - Mouse anti-CD25 FITC Flow Cytometry of Mouse anti-CD25 Fluorescein Conjugated Monoclonal Antibody. (CD25 抗体 (FITC))

FACS

1 image

(2 references)

产品编号 ABIN6655112

发货至: 中国

Datasheet as PDF

抗原 See all CD25 (IL2RA) 抗体

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
适用
165

99

35

9

6

3

3

2

1

1

1

1
人
宿主
125

78

70

2

1
小鼠
Compatible Secondaries
克隆类型
196

76

2
单克隆
标记
103

28

26

23

18

9

6

5

4

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD25 antibody is conjugated to FITC
应用范围
190

85

49

47

38

28

19

15

14

14

8

6

4

4

3

2

2

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (IHC)

交叉反应（详细）

Cross reactivity with CD25 from other sources has not been tested

纯化方法

Fluorescein conjugated CD25 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD25. Reactivity is observed against human CD25, Baboon, Chimpanzee, Cynomolgus, Rhesus, and Pigtailed Macaque. . Anti-CD25 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

免疫原

Immunogen: Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with CD25 antigen.

克隆位点

BC96

亚型

IgG1

Labeling Ratio

4-6

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应用备注

Immunohistochemistry Dilution: User Optimized
Application Note: Anti-CD25 is useful for Flow Cytometry and Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.
Flow Cytometry Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

限制

仅限研究用
状态

Liquid

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 0.2 % BSA (w/v)
0.09 % (w/v) Sodium Azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, (1993) (PubMed).

Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, (1991) (PubMed).
抗原

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

别名

CD25 (IL2RA 产品)

别名

CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody

背景

Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55
Background: CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
Gene Name: IL2RA

基因ID

3559

NCBI登录号

NP_000408

UniProt

P01589

途径

JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation