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CD25 抗体 (FITC)

Cited in 2+ publications. The FITC-conjugated 小鼠 单克隆 anti-CD25 antibody (Clone BC96) (ABIN6655112) specifically detects CD25 in FACS 和 IHC. The antibody is reactive with 人 samples.
Rockland
产品编号 ABIN6655112
Supplier Product No.: 200-302-n25
发货至: 中国
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Quick Overview for CD25 抗体 (FITC) (ABIN6655112)

抗原

See all CD25 (IL2RA) 抗体
CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

适用

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小鼠

克隆类型

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单克隆

标记

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This CD25 antibody is conjugated to FITC

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC)

克隆位点

BC96
  • Supplier Product No.

    200-302-n25

    Supplier

    Rockland

    原理

    CD25 Fluorescein Antibody

    交叉反应 (详细)

    Reactivity is observed against human CD25, Baboon, Chimpanzee, Cynomolgus, Rhesus, and Pigtailed Macaque.

    纯化方法

    Fluorescein conjugated CD25 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD25.

    过滤

    Sterile filtered

    免疫原

    Anti-CD25 Antibody (Monoclonal) was produced by repeated immunizations with CD25 antigen.

    亚型

    IgG1 kappa

    Labeling Ratio

    4-6
  • 应用备注

    Immunohistochemistry_Dilution: User Optimized

    Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

    说明

    Anti-CD25 is tested for Flow Cytometry and is useful for Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 0.2 % BSA (w/v)

    Preservative: 0.09 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

    有效期

    6 months
  • Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, (1993) (PubMed).

    Waldmann: "The interleukin-2 receptor." in: The Journal of biological chemistry, Vol. 266, Issue 5, pp. 2681-4, (1991) (PubMed).

  • 抗原

    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

    别名

    CD25

    背景

    Synonyms: Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55

    Background: CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

    Gene Name: IL2RA

    基因ID

    3559

    NCBI登录号

    NP_000408

    UniProt

    P01589

    途径

    JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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