Cyclin L1 抗体 (AA 314-369)
Quick Overview for Cyclin L1 抗体 (AA 314-369) (ABIN6655105)
抗原
See all Cyclin L1 (CCNL1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 314-369
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Supplier Product No.
- 200-401-881
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Supplier
- Rockland
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原理
- Cyclin L1 Antibody
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交叉反应 (详细)
- This antibody is specific for human Cyclin L1a and shows negligible reactivity to Cyclin L1b or Cyclin L2 isoforms.
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纯化方法
- The product was purified from monospecific antiserum by Protein A chromatography.
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免疫原
- This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to an internal region near amino acids 300-375 of Human Cyclin L1a protein.
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亚型
- IgG
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应用备注
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Immunoprecipitation_Dilution: 1:100
ELISA_Dilution: 1:5,000 - 1:25,000
Immunohistochemistry_Dilution: 10 mg/mL - 20 μg/mL
Western_Blot_Dilution: 1:2,000 - 1:5,000
Other: User Optimized
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说明
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This Protein A purified antibody has been tested for use in ELISA, immunohistochemistry, western blot, and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 59 kDa in size corresponding to Cyclin L1a by western blotting in the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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Role of CDK/cyclin complexes in transcription and RNA splicing." in: Cellular signalling, Vol. 17, Issue 9, pp. 1033-51, (2005) (PubMed).
: "
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Role of CDK/cyclin complexes in transcription and RNA splicing." in: Cellular signalling, Vol. 17, Issue 9, pp. 1033-51, (2005) (PubMed).
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- Cyclin L1 (CCNL1)
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别名
- Cyclin L1
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背景
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Synonyms: rabbit anti-Cyclin L1 antibody, Cyclin-L, Cyclin L beta, Cyclin L gamma, CCNL1
Background: Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively). Cyclin L has been shown to associate with the PITSLRE kinase and is involved in pre-mRNA processing. Both Cyclin L gene products can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.
Gene Name: CCNL1
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基因ID
- 57018
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NCBI登录号
- NP_064703
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UniProt
- Q9UK58
抗原
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