Nkx2-2 抗体
Quick Overview for Nkx2-2 抗体 (ABIN6654699)
抗原
See all Nkx2-2 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Purified
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纯度
- Protein G affinity chromatography
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免疫原
- Full length human recombinant protein was used as the immunogen for this recombinant NKX2.2 antibody.
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亚型
- IgG1 kappa
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant NKX2.2 antibody to be titered up or down for optimal performance.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
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限制
- 仅限研究用
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the recombinant NKX2.2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))
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别名
- NKX2.2
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背景
- Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. Antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors.
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途径
- Dopaminergic Neurogenesis
抗原
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