Cyclin E1 抗体 (AA 10-176)
Our Local Distributor
北京 101111
Quick Overview for Cyclin E1 抗体 (AA 10-176) (ABIN6654284)
抗原
See all Cyclin E1 (CCNE1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 10-176
-
纯化方法
- Purified
-
纯度
- Protein G affinity chromatography
-
免疫原
- Recombinant human partial protein (within amino acids 10-176) was used as the immunogen for the Cyclin E1 antibody.
-
亚型
- IgG
-
-
-
-
应用备注
-
Optimal dilution of the Cyclin E1 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT -
限制
- 仅限研究用
-
-
-
缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store the Cyclin E1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
-
-
- Cyclin E1 (CCNE1)
-
别名
- Cyclin E1 / CCNE1
-
背景
- Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.
-
途径
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases
抗原
-