MYLK 抗体
Quick Overview for MYLK 抗体 (ABIN6654097)
抗原
See all MYLK 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein A affinity
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免疫原
- A synthetic peptide from human Myosin light chain kinase was used as the immunogen for the MLCK antibody.
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亚型
- IgG
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anti-Myosin Light Chain Kinase (MYLK) (AA 1375-1524) antibody
MYLK 适用: 人, 小鼠 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 7F2B11 unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 1684-1884) antibodyMYLK 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 1441-1709) antibodyMYLK 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 1765-1914) antibodyMYLK 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 923-953), (N-Term) antibodyMYLK 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB03067 unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 908-938) antibodyMYLK 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 1661-1877) antibodyMYLK 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 1-992) antibodyMYLK 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (C-Term) antibodyMYLK 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Myosin Light Chain Kinase (MYLK) (AA 290-398) antibodyMYLK 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal dilution of the MLCK antibody should be determined by the researcher.\. Western Blot: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL
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限制
- 仅限研究用
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缓冲液
- Antibody in PBS with 0.02 % sodium azide and 50 % glycerol
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store the MLCK antibody at -20°C.
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- MYLK (Myosin Light Chain Kinase (MYLK))
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别名
- MYLK / MLCK
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背景
- Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA- dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury, triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca(2+) entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells. Responsible for high proliferative ability of breast cancer cells through anti-apoptosis. .
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UniProt
- Q15746
抗原
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