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beta Actin 抗体

Name: beta Actin 抗体
SKU: ABIN658990
Availability: OutOfStock

This anti-beta Actin antibody is a 小鼠单克隆 antibody detecting beta Actin in WB, IF, IHC (p), FACS 和 DNA Mic. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 12+ publications.

All lanes : Anti-ACTB Antibody at 1:2000 dilution + C6 cell lysate Lysates/proteins at 20 μg per lane. (beta Actin 抗体)

Immunohistochemical analysis of paraffin-embedded H. (beta Actin 抗体)

IHC (p)

Confocal immunofluorescent analysis of ACTB Antibody (ABIN658990 and ABIN2838035) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). (beta Actin 抗体)

All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: C6 whole cell lysate Lane 4: Hela whole cell lysate Lane 5: MCF-7 whole cell lysate Lysates/proteins at 20 μg per lane. (beta Actin 抗体)

Western blot analysis of anti-ACTB Antibody (ABIN658990 and ABIN2838035) in K562, HL-60,Hela cell line, mouse spleen, mouse liver tissue lysates, mouse NIH-3T3 cell line lysate and mouse cerebellum, mouse brain tissue lysates (35 μg/lane). (beta Actin 抗体)

All lanes : Anti-ACTB Antibody at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: NIH-3T3 whole cell lysate Lysates/proteins at 20 μg per lane. (beta Actin 抗体)

IHC (p)

7 images

(12 references)

产品编号 ABIN658990

发货至: 中国

Datasheet as PDF

Quick Overview for beta Actin 抗体 (ABIN658990)

抗原

See all beta Actin (ACTB) 抗体

beta Actin (ACTB) (Actin, beta (ACTB))

适用

人, 小鼠, 大鼠

宿主

小鼠

克隆类型

单克隆

标记

This beta Actin antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), DNA Microscopy (DNA Mic)

克隆位点

137CT26-1-1

Discover our top product ACTB Primary Antibody

预测反应

X, B, C, Hs, Pr, Pig, Sh

纯化方法

This antibody is purified through a protein G column, followed by dialysis against PBS.

免疫原

This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein.

亚型

IgG1, Ig kappa
anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人, 小鼠, 大鼠, 兔, Pig, Cow WB, ELISA, IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 2-16) antibody
ACTB 适用: 小鼠, 大鼠, 小鸡, 斑马鱼 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 350-375) antibody
ACTB 适用: 人, 小鼠, Leopard Frog WB, ELISA, IF, FM 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB 适用: 人, 小鼠, 大鼠, Pig, Cow WB, IHC (p) 宿主: 小鼠 Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB 适用: 人, 小鼠, 小鸡, 兔, Pig, Cow, Hamster, 山羊, 鱼 WB, ELISA 宿主: 小鼠 Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB 适用: 人 WB, ELISA, IHC, IF, IP, FACS 宿主: 小鼠 Monoclonal 1D12E12B5 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
ACTB 适用: 人, 小鼠, 大鼠, 小鸡, 兔, 犬, 猴, Pig, Cow, 绵羊, Hamster, 马, 豚鼠, 山羊, Cat, Avian, 驴 WB, IF 宿主: 山羊 Polyclonal unconjugated

anti-Actin, beta (ACTB) (Middle Region) antibody
ACTB 适用: 人, 小鼠, 大鼠, Pig, Cow, 绵羊, 斑马鱼, 马, 山羊 WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:10~50. WB: 1:1000. WB: 1:1000. WB: 1:1000. IHC-P: 1:25. IHC-P: 1:10~50. IHC-P: 1:25

限制

仅限研究用
状态

Liquid

缓冲液

Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Lin, Chen, Huang, Xu: "CYLD Promotes TNF-α-Induced Cell Necrosis Mediated by RIP-1 in Human Lung Cancer Cells." in: Mediators of inflammation, Vol. 2016, pp. 1542786, (2017) (PubMed).

Munoz, Yazdi, Tang, Rivera, Taghipour, Grossman, Boone, Tasciotti: "Localized inhibition of P2X7R at the spinal cord injury site improves neurogenic bladder dysfunction by decreasing urothelial P2X3R expression in rats." in: Life sciences, Vol. 171, pp. 60-67, (2017) (PubMed).

Vispute, Bu, Le, Cheng: "Activation of GR but not PXR by dexamethasone attenuated acetaminophen hepatotoxicities via Fgf21 induction." in: Toxicology, Vol. 378, pp. 95-106, (2017) (PubMed).

Bu, Le, Zhang, Zhang, Cheng: "Berberine-induced Inactivation of Signal Transducer and Activator of Transcription 5 Signaling Promotes Male-specific Expression of a Bile Acid Uptake Transporter." in: The Journal of biological chemistry, Vol. 292, Issue 11, pp. 4602-4613, (2017) (PubMed).

Lau, Tang, Casey, Wang: "Isoprenylcysteine carboxylmethyltransferase is critical for malignant transformation and tumor maintenance by all RAS isoforms." in: Oncogene, Vol. 36, Issue 27, pp. 3934-3942, (2017) (PubMed).

Martins, Bung, Koch, Richter, Schwarzmüller, Terhardt, Kurtulmus, Niehrs, Rouhi, Lohmann, Pereira, Fröhling, Glimm, Scholl: "Stk33 is required for spermatid differentiation and male fertility in mice." in: Developmental biology, Vol. 433, Issue 1, pp. 84-93, (2017) (PubMed).

Wei, Ma, Ding, Jin, Liu, Chang, Hu, Yu: "Reduced Glutamate Release in Adult BTBR Mouse Model of Autism Spectrum Disorder." in: Neurochemical research, Vol. 41, Issue 11, pp. 3129-3137, (2016) (PubMed).

Harada, Katsuki, Takahashi, Masuda, Yoshinaga, Adachi, Izawa, Kuwamura, Nakano, Yamaji, Inui: "Androgen receptor silences thioredoxin-interacting protein and competitively inhibits glucocorticoid receptor-mediated apoptosis in pancreatic ?-Cells." in: Journal of cellular biochemistry, Vol. 116, Issue 6, pp. 998-1006, (2015) (PubMed).

Koutakis, Miserlis, Myers, Kim, Zhu, Papoutsi, Swanson, Haynatzki, Ha, Carpenter, McComb, Johanning, Casale, Pipinos et al.: "Abnormal accumulation of desmin in gastrocnemius myofibers of patients with peripheral artery disease: associations with altered myofiber morphology and density, mitochondrial dysfunction and ..." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 63, Issue 4, pp. 256-69, (2015) (PubMed).

Vatter, Di, Donaldson, Radu, Maines, Brinton: "Functional analyses of the three simian hemorrhagic fever virus nonstructural protein 1 papain-like proteases." in: Journal of virology, Vol. 88, Issue 16, pp. 9129-40, (2014) (PubMed).

Miyazawa, Tsuji: "Evidence for a novel antioxidant function and isoform-specific regulation of the human p66Shc gene." in: Molecular biology of the cell, Vol. 25, Issue 13, pp. 2116-27, (2014) (PubMed).

Choi, Li, Felsher: "Addiction to multiple oncogenes can be exploited to prevent the emergence of therapeutic resistance." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 32, pp. E3316-24, (2014) (PubMed).
抗原

beta Actin (ACTB) (Actin, beta (ACTB))

别名

ACTB

背景

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

分子量

41737

基因ID

60

NCBI登录号

NP_001092

UniProt

P60709

途径

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction