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SMAD3 抗体 (AA 186-215)

Name: SMAD3 抗体 (AA 186-215)
SKU: ABIN653953
Availability: OutOfStock

SMAD3 适用: 人, 小鼠 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB24758 unconjugated

SD3 Antibody (ABIN653953 and ABIN2843175) western blot analysis in HepG2 and mouse C2C12 cell line lysates (35 μg/lane). (SMAD3 抗体 (AA 186-215))

SD3- Antibody (ABIN653953 and ABIN2843175) flow cytometric analysis of MDA-M cells (right histogram) compared to a negative control cell (left histogram). (SMAD3 抗体 (AA 186-215))

FACS

Confocal immunofluorescent analysis of SD3- Antibody (ABIN653953 and ABIN2843175) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (SMAD3 抗体 (AA 186-215))

SD3- Antibody (cat. (SMAD3 抗体 (AA 186-215))

IHC (p)

4 images

(2 references)

产品编号 ABIN653953

发货至: 中国

Datasheet as PDF

抗原 See all SMAD3 抗体

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
抗原表位
34

29

28

28

20

14

13

9

8

7

6

6

5

5

5

4

4

4

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 186-215
适用
243

149

138

28

10

9

8

8

7

5

4

3

3

2

1

1

1
人, 小鼠
宿主
234

23
兔
Compatible Secondaries
克隆类型
217

40
多克隆
标记
136

17

14

13

10

7

6

6

6

6

6

6

4

4

4

4

4

1

1

1

1
This SMAD3 antibody is un-conjugated
应用范围
217

117

95

73

58

55

54

53

28

13

10

8

5

4

2

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

预测反应

C, Pig, Rat

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This SMAD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-215 amino acids from human SMAD3.

克隆位点

RB24758

亚型

Ig Fraction

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anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 417-425), (pSer423), (pSer425) antibody
SMAD3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 7F3 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-230) antibody
SMAD3 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2C12 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-110) antibody
SMAD3 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C11 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C12 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) antibody
SMAD3 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS, IF (cc) 宿主: 兔 Monoclonal 3D1 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 231-425) antibody
SMAD3 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (C-Term) antibody
SMAD3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (pThr179) antibody
SMAD3 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Zhou, Liu, Pan, Du, Li, Ma, Yao, Li, Liu: "The let-7g microRNA promotes follicular granulosa cell apoptosis by targeting transforming growth factor-? type 1 receptor." in: Molecular and cellular endocrinology, Vol. 409, pp. 103-12, (2015) (PubMed).

Lessard, Rivas, Alves-Wagner, Hirshman, Gallagher, Constantin-Teodosiu, Atkins, Greenhaff, Qi, Gustafsson, Fielding, Timmons, Britton, Koch, Goodyear: "Resistance to aerobic exercise training causes metabolic dysfunction and reveals novel exercise-regulated signaling networks." in: Diabetes, Vol. 62, Issue 8, pp. 2717-27, (2013) (PubMed).
抗原

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

别名

SMAD3 (SMAD3 产品)

别名

HSPC193 antibody, HsT17436 antibody, JV15-2 antibody, LDS1C antibody, LDS3 antibody, MADH3 antibody, madh3 antibody, madh3a antibody, smad3 antibody, wu:fa99e03 antibody, XSmad3 antibody, XenMLP antibody, Xmad3 antibody, madh3-A antibody, Madh3 antibody, AU022421 antibody, Smad 3 antibody, mad3 antibody, madh3b antibody, zgc:92234 antibody, SMAD family member 3 antibody, SMAD family member 3a antibody, SMAD family member 3 L homeolog antibody, SMAD family member 3b antibody, SMAD3 antibody, smad3a antibody, smad3.L antibody, Smad3 antibody, smad3b antibody

背景

SMAD3 belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

分子量

48081

基因ID

4088

NCBI登录号

NP_001138574, NP_001138575, NP_001138576, NP_005893

UniProt

P84022

途径

Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy