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SMAD3 抗体 (pSer213)

Name: SMAD3 抗体 (pSer213)
SKU: ABIN389657
Availability: OutOfStock

SMAD3 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB29526 unconjugated

The anti-Phospho-SD3- Pab (ABIN389657 and ABIN2839640) is used in Western blot to detect Phospho-SD3- in Ramos tissue lysate (SMAD3 抗体 (pSer213))

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (SMAD3 抗体 (pSer213))

IHC (p)

All lanes : Anti-SD3 Antibody at 1:1000 dilution Lane 1: HACAT whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: Hela whole cell lysate Lysates/proteins at 20 μg per lane. (SMAD3 抗体 (pSer213))

3 images

(1 reference)

产品编号 ABIN389657

发货至: 中国

Datasheet as PDF

抗原 See all SMAD3 抗体

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
抗原表位
32

28

27

26

20

14

13

8

8

7

6

6

5

5

5

4

4

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer213
适用
234

146

134

28

10

9

8

8

7

5

3

3

2

2

1

1

1
人
宿主
225

23
兔
Compatible Secondaries
克隆类型
211

37
多克隆
标记
127

17

14

13

10

7

6

6

6

6

6

6

4

4

4

4

4

1

1

1

1
This SMAD3 antibody is un-conjugated
应用范围
209

113

94

74

56

55

53

49

26

13

10

8

5

4

2

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

预测反应

C, M, Pig, Rat

纯化方法

This antibody is purified through a protein A column, followed by peptide affinity purification.

免疫原

This SMAD3 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S213 of human SMAD3.

克隆位点

RB29526

亚型

Ig Fraction

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SMAD3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 7F3 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-230) antibody
SMAD3 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2C12 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-110) antibody
SMAD3 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1C11 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) antibody
SMAD3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2C12 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) antibody
SMAD3 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS, IF (cc) 宿主: 兔 Monoclonal 3D1 unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 231-425) antibody
SMAD3 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (C-Term) antibody
SMAD3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-SMAD, Mothers Against DPP Homolog 3 (SMAD3) (pThr179) antibody
SMAD3 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100

限制

仅限研究用
状态

Liquid

缓冲液

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

有效期

6 months
Wu, Bian, Dou, Gong, Tan, Xia, Dai: "Asiaticoside hinders the invasive growth of keloid fibroblasts through inhibition of the GDF-9/MAPK/Smad pathway." in: Journal of biochemical and molecular toxicology, Vol. 31, Issue 8, (2017) (PubMed).
抗原

SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

别名

SMAD3 (SMAD3 产品)

别名

HSPC193 antibody, HsT17436 antibody, JV15-2 antibody, LDS1C antibody, LDS3 antibody, MADH3 antibody, madh3 antibody, madh3a antibody, smad3 antibody, wu:fa99e03 antibody, XSmad3 antibody, XenMLP antibody, Xmad3 antibody, madh3-A antibody, Madh3 antibody, AU022421 antibody, Smad 3 antibody, mad3 antibody, madh3b antibody, zgc:92234 antibody, SMAD family member 3 antibody, SMAD family member 3a antibody, SMAD family member 3 L homeolog antibody, SMAD family member 3b antibody, SMAD3 antibody, smad3a antibody, smad3.L antibody, Smad3 antibody, smad3b antibody

背景

SMAD3, a receptor regulated SMAD (R-SMAD) is a transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinase. SMAD3 is estimated to account for at least 80 % of all TGF-beta-mediated response. Activated type I receptor phosphorylates receptor-activated SMADS (RSMADS) at their c-terminal two extreme serines in the SSXS motif. The phosphorylated R-SMAD translocate into nucleus, where they regulate transcription of target genes. SMAD3 signal transduction appears to be important in the rgulation of muscle-specific genes. Loss of SMAD3 is a feature of pediatric T-cell lymphoblastic leukemia, while upregulation of SMAD3 may be responsible for TGFB hyperresponsiveness observed in scleroderma.

分子量

48081

基因ID

4088

NCBI登录号

NP_001138574, NP_001138575, NP_001138576, NP_005893

UniProt

P84022

途径

Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy