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Sonic Hedgehog 抗体 (C-Term)

This anti-Sonic Hedgehog antibody is a 山羊 多克隆 antibody detecting Sonic Hedgehog in WB, ELISA, IF 和 FACS. Suitable for 人 和 小鼠.
产品编号 ABIN6391361
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
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Quick Overview for Sonic Hedgehog 抗体 (C-Term) (ABIN6391361)

抗原

See all Sonic Hedgehog (SHH) 抗体
Sonic Hedgehog (SHH)

适用

  • 87
  • 60
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  • 2
  • 2
  • 1
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  • 1
人, 小鼠

宿主

  • 91
  • 14
  • 14
  • 1
山羊

克隆类型

  • 72
  • 48
多克隆

标记

  • 62
  • 11
  • 5
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This Sonic Hedgehog antibody is un-conjugated

应用范围

  • 87
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  • 17
  • 14
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  • 10
  • 10
  • 5
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Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

质量等级

Verified
  • 抗原表位

    • 15
    • 12
    • 9
    • 5
    • 5
    • 4
    • 4
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    • 2
    • 2
    • 2
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    C-Term

    原理

    SHH

    序列

    DSEALHPLGM AVK

    交叉反应

    犬, 人, 大鼠

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    亚型

    IgG
  • 应用备注

    Western Blot: Approx 48 kDa band observed in Human Liver lysates (calculated MW of 49.6 kDa according to NP_000184.1 Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Liver for t

    Peptide ELISA: antibody detection limit dilution 1:32000.

    说明

    Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa, U251 and NIH3T3 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concen

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    Sonic Hedgehog (SHH)

    别名

    SHH

    背景

    SHH, sonic hedgehog homolog (Drosophila), HGNC:10848, HHG1, HLP3, HPE3, SMMCI , sonic hedgehog

    基因ID

    6469

    NCBI登录号

    NP_000184

    途径

    Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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