Sonic Hedgehog 抗体 (C-Term)
Quick Overview for Sonic Hedgehog 抗体 (C-Term) (ABIN6391361)
抗原
See all Sonic Hedgehog (SHH) 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- C-Term
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原理
- SHH
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序列
- DSEALHPLGM AVK
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交叉反应
- 犬, 人, 大鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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亚型
- IgG
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anti-Sonic Hedgehog (SHH) (AA 21-120) antibody
SHH 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Sonic Hedgehog (SHH) (AA 26-161) antibodySHH 适用: 人, 小鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5H4 unconjugated
anti-Sonic Hedgehog (SHH) (N-Term) antibodySHH 适用: 人, 小鼠, 大鼠, 斑马鱼, 犬, Cow, 豚鼠, 马, 山羊, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sonic Hedgehog (SHH) (AA 26-161) antibodySHH 适用: 人, 小鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal unconjugated
anti-Sonic Hedgehog (SHH) (AA 36-193) antibodySHH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sonic Hedgehog (SHH) (AA 36-193) antibodySHH 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Sonic Hedgehog (SHH) (AA 58-91), (N-Term) antibodySHH 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB52387 unconjugated
anti-Sonic Hedgehog (SHH) (AA 24-197) antibodySHH 适用: 人, 大鼠, 犬, 猴 WB, IF 宿主: 小鼠 Monoclonal 10H6 unconjugated
anti-Sonic Hedgehog (SHH) (AA 203-218), (N-Term) antibodySHH 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sonic Hedgehog (SHH) (AA 237-399) antibodySHH 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western Blot: Approx 48 kDa band observed in Human Liver lysates (calculated MW of 49.6 kDa according to NP_000184.1 Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Liver for t
Peptide ELISA: antibody detection limit dilution 1:32000.
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说明
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Immunofluorescence: Strong expression of the protein seen in the membranes of HeLa, U251 and NIH3T3 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concen
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Sonic Hedgehog (SHH)
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别名
- SHH
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背景
- SHH, sonic hedgehog homolog (Drosophila), HGNC:10848, HHG1, HLP3, HPE3, SMMCI , sonic hedgehog
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基因ID
- 6469
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NCBI登录号
- NP_000184
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途径
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
抗原
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