我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

Sonic Hedgehog 抗体 (AA 26-161)

Name: Sonic Hedgehog 抗体 (AA 26-161)
Brand: antibodies-online
SKU: ABIN969567
Availability: OutOfStock

SHH 适用: 人, 小鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5H4 unconjugated

Western blot analysis using SHH mouse mAb against LNCaP (1), HepG2 (2), PANC-1 (3),HeLa (4), SK-N-SH (5), F9 (6), NIH3T3 (7), and COS7 (8) cell lysate.

Western blot analysis using SHH mAb against human SHH recombinant protein.

Western blot analysis using SHH mAb against HEK293 (1) and SHH (AA: 26-161)-hIgGFc transfected HEK293 (2) cell lysate.

Black line: Control Antigen (100 ng), Purple line: Antigen(10 ng), Blue line: Antigen (50 ng), Red line: Antigen (100 ng),

ELISA

Flow cytometric analysis of HeLa cells using SHH mouse mAb (green) and negative control (red).

FACS

Immunohistochemical analysis of paraffin-embedded liver cancer tissues using SHH mouse mAb with DAB staining.

IHC

6 images

(1 reference)

产品编号 ABIN969567

发货至: 中国

Datasheet as PDF

抗原 See all Sonic Hedgehog (SHH) 抗体

Sonic Hedgehog (SHH)
抗原表位
15

11

7

6

4

4

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
AA 26-161
适用
60

53

40

17

9

7

6

5

2

2

2

1

1

1

1

1

1

1
人, 小鼠, 猴
宿主
73

9

8

1

1
小鼠
Compatible Secondaries
克隆类型
66

26
单克隆
标记
52

10

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This Sonic Hedgehog antibody is un-conjugated
应用范围
72

31

24

14

13

11

10

7

7

7

5

4

3

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

纯化方法

purified

免疫原

Purified recombinant fragment of human SHH (AA: 26-161) expressed in E. coli. ,

克隆位点

5H4

亚型

IgG1

Top Product

Discover our top product SHH Primary Antibody
anti-Sonic Hedgehog (SHH) (AA 21-120) antibody
SHH 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (C-Term) antibody
Verified SHH 适用: 人, 小鼠 WB, ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (N-Term) antibody
SHH 适用: 人, 小鼠, 大鼠, 斑马鱼, Cow, 犬, 马, 豚鼠, 山羊, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (AA 36-193) antibody
SHH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (AA 36-193) antibody
SHH 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D6 unconjugated

anti-Sonic Hedgehog (SHH) (AA 58-91), (N-Term) antibody
SHH 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB52387 unconjugated

anti-Sonic Hedgehog (SHH) (AA 24-197) antibody
SHH 适用: 人, 大鼠, 犬, 猴 WB, IF 宿主: 小鼠 Monoclonal 10H6 unconjugated

anti-Sonic Hedgehog (SHH) (AA 203-218), (N-Term) antibody
SHH 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (AA 237-399) antibody
SHH 适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated

anti-Sonic Hedgehog (SHH) (AA 28-193) antibody
SHH 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400

限制

仅限研究用
状态

Liquid

缓冲液

Purified antibody in PBS with 0.05 % sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

4°C, -20°C for long term storage
Kameda, Nakamura, Tanaka, Yamasaki, Kubo, Tanaka, Onishi, Katano: "Oestrogen receptor-alpha contributes to the regulation of the hedgehog signalling pathway in ERalpha-positive gastric cancer." in: British journal of cancer, Vol. 102, Issue 4, pp. 738-47, (2010) (PubMed).
抗原

Sonic Hedgehog (SHH)

别名

SHH (SHH 产品)

别名

HHG1 antibody, HLP3 antibody, HPE3 antibody, MCOPCB5 antibody, SMMCI antibody, TPT antibody, TPTPS antibody, 9530036O11Rik antibody, Dsh antibody, Hhg1 antibody, Hx antibody, Hxl3 antibody, M100081 antibody, fc83d08 antibody, shh antibody, syu antibody, vhh-1 antibody, vhh1 antibody, wu:fc83d08 antibody, Xhh antibody, hedgehog antibody, xshh antibody, SHH antibody, twh antibody, twhh antibody, sonic hedgehog antibody, sonic hedgehog a antibody, sonic hedgehog L homeolog antibody, sonic hedgehog protein A antibody, sonic hedgehog b antibody, SHH antibody, Shh antibody, shha antibody, shh.L antibody, shh antibody, shhb antibody

背景

Description: This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved, the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.
Aliases: TPT, HHG1, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5

分子量

49.6 kDa

基因ID

6469

HGNC

6469

途径

Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development