RFC5 抗体
Quick Overview for RFC5 抗体 (ABIN634103)
抗原
See all RFC5 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Affinity purified
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免疫原
- RFC5 antibody was raised using a synthetic peptide corresponding to a region with amino acids METSALKQQEQPAATKIRNLPWVEKYRPQTLNDLISHQDILSTIQKFINE
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应用备注
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WB: 1 µg/mL, IHC: 4-8 µg/mL
Optimal conditions should be determined by the investigator. -
说明
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RFC5 Blocking Peptide, catalog no. 33R-5977, is also available for use as a blocking control in assays to test for specificity of this RFC5 antibody
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Lyophilized powder. Add distilled water for a 1 mg/mL concentration of RFC5 antibody in PBS
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浓度
- Lot specific
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缓冲液
- PBS
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注意事项
- Avoid repeated freeze/thaw cycles.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.
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- RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa (RFC5))
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别名
- RFC5
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背景
- The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. RFC5 is the 36 kDa subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system.
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分子量
- 38 kDa (MW of target protein)
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途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
抗原
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