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Crossover junction endonuclease EME1 (EME1) 抗体

The 兔 多克隆 anti-Crossover junction endonuclease EME1 antibody is suitable to detect Crossover junction endonuclease EME1 in samples from 人 和 小鼠. It has been validated for WB.
产品编号 ABIN631739
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Crossover junction endonuclease EME1 (EME1) 抗体 (ABIN631739)

抗原

See all Crossover junction endonuclease EME1 (EME1) 抗体
Crossover junction endonuclease EME1 (EME1)

适用

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人, 小鼠

宿主

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克隆类型

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多克隆

标记

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非结合性

应用范围

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Western Blotting (WB)
  • 纯化方法

    Affinity purified

    免疫原

    EME1 antibody was raised using a synthetic peptide corresponding to a region with amino acids AYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEGVTSTSRRIGPELSRR
  • 应用备注

    WB: 1 µg/mL
    Optimal conditions should be determined by the investigator.

    说明

    EME1 Blocking Peptide, (ABIN937018), is also available for use as a blocking control in assays to test for specificity of this EME1 antibody

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Lyophilized powder. Add distilled water for a 1 mg/mL concentration of EME1 antibody in PBS

    浓度

    Lot specific

    缓冲液

    PBS

    注意事项

    Avoid repeated freeze/thaw cycles.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.
  • 抗原

    Crossover junction endonuclease EME1 (EME1)

    别名

    EME1

    背景

    EME1 interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. EME1 may be required in mitosis for the processing of stalled or collapsed replication forks.EME1 and MUS81 form an endonuclease complex that cleaves branched DNA structures, especially those arising during stalled DNA replication.

    分子量

    63 kDa (MW of target protein)
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