S100A8 抗体
Quick Overview for S100A8 抗体 (ABIN6296169)
抗原
See all S100A8 抗体适用
宿主
克隆类型
标记
应用范围
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原理
- Mouse anti-Human/Baboon/Monkey/Cow/Pig/Goat/Horse/Cat/Dog/Rabbit/Guinea Pig/Rat/Mouse Macrophage Antibody [Sodium Azide Free]
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特异性
- Cytoplasmic
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纯化方法
- An affinity purified monocyte membrane preparation was used as the immunogen for the anti-Macrophage antibody.
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免疫原
- An affinity purified monocyte membrane preparation was used as the immunogen for the anti-Macrophage antibody.
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1) -
限制
- 仅限研究用
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缓冲液
- In 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- S100A8 (S100 Calcium Binding Protein A8 (S100A8))
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别名
- Protein S100-A8
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背景
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Target Description: Recognizes the macrophage L1 protein Calprotectin, expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This mAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections.
Gene Symbol: S100A8///CAGA///CFAG///MRP8///S100A9///CAGB///CFAG///MRP14
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UniProt
- P05109, P06702
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途径
- Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
抗原
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