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Mitochondria 抗体

This 小鼠 单克隆 antibody specifically detects Mitochondria in IHC, WB 和 IF. It exhibits reactivity toward 人.
产品编号 ABIN6296107
发货至: 中国
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Quick Overview for Mitochondria 抗体 (ABIN6296107)

抗原

Mitochondria

适用

宿主

  • 12
  • 6
  • 1
小鼠

克隆类型

  • 17
  • 2
单克隆

标记

  • 11
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
非结合性

应用范围

  • 15
  • 10
  • 5
  • 5
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)
  • 原理

    Mouse anti-Human Mitochondria Antibody [Sodium Azide Free]

    特异性

    Mitochondria in cytoplasm

    纯化方法

    A semi-purified mitochondria preparation was used as the immunogen for the Mitochondria antibody.

    免疫原

    A semi-purified mitochondria preparation was used as the immunogen for the Mitochondria antibody.
  • 应用备注

    Western blot: 1-2 μg/mL
    IF: 0.5-1 μg/mL
    IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
    Prediluted format: incubate for 30 min at RT (2)

    限制

    仅限研究用
  • 缓冲液

    In 1X PBS ( pH 7.4), BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • 抗原

    Mitochondria

    背景

    Target Description: Clone MTC02 antibody recognizes a unique 60 kDa antigen that is present only on mitochondria in human cells. It is a part of a panel of reagents which recognizes subcellular organelles or compartments. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This antibody recognizes an antigen associated with the mitochondria specific to human cells alone. MTC02 mAb can be used to stain the mitochondria in cell or tissue preparations and can be used as a marker in subcellular fractions. The antibody produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. MTC02 antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.

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