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- 抗原
- Mitochondria
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)
- 纯化方法
- Anti-Mitochondria antibody is specific for human mitochondria in all cell types. The antibody is a protein A purification from concentrated clarified cell culture supernate. The antibody reacts specifically with human cells, including neurons and embryonic stem cells. The antibody does not crossreact with mitochondria from mouse or rat.
- 免疫原
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Immunogen: Anti-Mitochondria Monoclonal Antibody was produced by repeated immunizations with a human cell homogenate.
Immunogen Type: Other
- 克隆位点
- 113-1
- 亚型
- IgG1
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anti-Mitochondria antibody
适用: 人 WB, IF, IHC (p), ELISA 宿主: 小鼠 Monoclonal AE-1 unconjugated
anti-Mitochondria antibody适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal AE-1 unconjugated
anti-Mitochondria antibody适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal SPM198 unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:800
Application Note: Anti-Mitochondria antibody is suitable for use in Western Blotting, IHC and IF. Expect ~60 kDa in WB. Anti-Anti-Mitochondria antibody works on acetone fixed and 2 % paraformaldehyde fixed cells. The antibody also works for immunohistochemistry on 2 % paraformaldehyde fixed cryostat sections. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:10,000 - 1:40,000
Western Blot Dilution: 1:200 - 1:2,000
IF Microscopy Dilution: 1:100 - 1:1,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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- 抗原
- Mitochondria
- 背景
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Synonyms: mouse anti-Mitochondria Antibody, Human cell marker antibody, xenographic model research antibodies, anti-mitochondria antibody produced in mouse
Background: Mitochondria antibody detects human Mitochondria. Mitochonria are most commonly known as the power plants of the cell as they produce ATP, but they are also involved in many other important cellular processes such as cell signaling, growth and differentiation. In addition, mitochondria have been shown to play a role in apoptosis. This antibody is an excellent marker for human cells in xenographic model research.
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