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- 抗原
- Mitochondria
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 无交叉反应
- 小鼠, 大鼠
- 产品特性
- This mAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. It reacts specifically with human cells, including neurons and embryonic stem cells.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondria antibody.
- 克隆位点
- SPM198
- 亚型
- IgG1 kappa
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anti-Mitochondria antibody
适用: 人 WB, IF, IHC (p), ELISA 宿主: 小鼠 Monoclonal AE-1 unconjugated
anti-Mitochondria antibody适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal AE-1 unconjugated
anti-Mitochondria antibody适用: 人 WB, IHC, ELISA, FM 宿主: 小鼠 Monoclonal 113-1 unconjugated
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- 应用备注
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Optimal dilution of the anti-Mitochondria antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.25-0.5 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the anti-Mitochondria antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Mitochondria
- 背景
- This mAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. It reacts specifically with human cells, including neurons and embryonic stem cells.
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