GJB1 抗体 (Cytoplasmic Loop)
Quick Overview for GJB1 抗体 (Cytoplasmic Loop) (ABIN6295447)
抗原
See all GJB1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Cytoplasmic Loop
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原理
- Mouse anti-Rat/Mouse/Human Connexin 32 Antibody / GJB1 [Sodium Azide Free]
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特异性
- Cell surface, cytoplasmic
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纯化方法
- Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).
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免疫原
- Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
WB: 0.5-1 μg/mL
IHC (Frozen): 0.5-1 μg/mL for 30 min at RT -
限制
- 仅限研究用
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缓冲液
- In 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))
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别名
- Gap junction beta-1 protein
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背景
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Target Description: This Ab recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.
Gene Symbol: GJB1///CX32
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基因ID
- 2705
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UniProt
- P08034
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途径
- Cell-Cell Junction Organization
抗原
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