CD3 epsilon 抗体 (Cytoplasmic Domain)
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Quick Overview for CD3 epsilon 抗体 (Cytoplasmic Domain) (ABIN6294325)
抗原
See all CD3 epsilon (CD3E) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 156-168, Cytoplasmic Domain
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原理
- Mouse anti-Human/Mouse/Rat/Rabbit/Guinea pig/Pig/Horse/Cow CD3e Antibody [Sodium Azide Free]
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特异性
- Cell surface and cytoplasmic
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纯化方法
- Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
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免疫原
- Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
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应用备注
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WB: 1-2 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 min at RT
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL -
限制
- 仅限研究用
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缓冲液
- In 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- CD3 epsilon (CD3E)
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别名
- T-cell surface glycoprotein CD3 epsilon chain
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背景
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Target Description: Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Gene Symbol: CD3E///T3E
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基因ID
- 916
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UniProt
- P07766
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途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
抗原
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