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CD3 epsilon 抗体 (Cytoplasmic Domain)

This 小鼠 单克隆 antibody specifically detects CD3 epsilon in FACS, WB, IHC 和 IF. It exhibits reactivity toward 人, 小鼠, 大鼠, Pig, Cow, 马, 兔 和 豚鼠.
产品编号 ABIN6294325
发货至: 中国
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Quick Overview for CD3 epsilon 抗体 (Cytoplasmic Domain) (ABIN6294325)

抗原

See all CD3 epsilon (CD3E) 抗体
CD3 epsilon (CD3E)

适用

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人, 小鼠, 大鼠, Pig, Cow, 马, 兔, 豚鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This CD3 epsilon antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
  • 抗原表位

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    AA 156-168, Cytoplasmic Domain

    原理

    Mouse anti-Human/Mouse/Rat/Rabbit/Guinea pig/Pig/Horse/Cow CD3e Antibody [Sodium Azide Free]

    特异性

    Cell surface and cytoplasmic

    纯化方法

    Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.

    免疫原

    Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
  • 应用备注

    WB: 1-2 μg/mL
    IHC (FFPE): 1-2 μg/mL for 30 min at RT
    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
    Immunofluorescence: 1-2 μg/mL

    限制

    仅限研究用
  • 缓冲液

    In 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
  • 抗原

    CD3 epsilon (CD3E)

    别名

    T-cell surface glycoprotein CD3 epsilon chain

    背景

    Target Description: Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

    Gene Symbol: CD3E///T3E

    基因ID

    916

    UniProt

    P07766

    途径

    TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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