UHRF1 抗体 (N-Term)
Quick Overview for UHRF1 抗体 (N-Term) (ABIN6265880)
抗原
See all UHRF1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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特异性
- UHRF1 Antibody detects endogenous levels of total UHRF1.
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预测反应
- Pig,Zebrafish,Bovine,Horse,Sheep,Dog,Chicken,Xenopus
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纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
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免疫原
- A synthesized peptide derived from human UHRF1, corresponding to a region within N-terminal amino acids.
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亚型
- IgG
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anti-Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) (AA 1-260) antibody
UHRF1 适用: 人 WB, IF, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) (AA 616-755) antibodyUHRF1 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 1A1B1 unconjugated
anti-Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) (AA 694-793) antibodyUHRF1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3A11 unconjugated
anti-Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) (AA 616-755) antibodyUHRF1 适用: 人 WB, ELISA, IHC 宿主: 小鼠 Monoclonal 2A8C7 unconjugated
anti-Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) (AA 229-257) antibodyUHRF1 适用: 人 WB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) antibodyKO Validated UHRF1 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) (AA 596-628), (C-Term) antibodyUHRF1 适用: 小鼠 WB 宿主: 兔 Polyclonal RB51453 unconjugated
anti-Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) (meLys385) antibodyUHRF1 适用: 人 WB, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) (AA 229-257) antibodyUHRF1 适用: 人 WB, IF, FACS 宿主: 兔 Polyclonal RB21516 unconjugated
anti-Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1) antibodyUHRF1 适用: 人 WB, FACS, IF/ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:2000, IHC 1:500-1:100000, IF/ICC 1:20-1:50, IP 1:20-1:50, CHIP 1:20-1:50, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- UHRF1 (Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1))
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别名
- UHRF1
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背景
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Description: Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. May be involved in DNA repair.
Gene: UHRF1
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分子量
- 91kDa
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基因ID
- 29128
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UniProt
- Q96T88
抗原
-
