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SF3B3 抗体 (C-Term)

This 兔 多克隆 antibody specifically detects SF3B3 in WB, ELISA, IF 和 ICC. It exhibits reactivity toward 人 和 小鼠.
产品编号 ABIN6257489
发货至: 中国
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Quick Overview for SF3B3 抗体 (C-Term) (ABIN6257489)

抗原

See all SF3B3 抗体
SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

适用

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人, 小鼠

宿主

  • 63
  • 2
  • 1

克隆类型

  • 37
  • 29
多克隆

标记

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This SF3B3 antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
  • 抗原表位

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    C-Term

    特异性

    SF3B3 Antibody detects endogenous levels of total SF3B3.

    预测反应

    Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Xenopus

    纯化方法

    The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).

    免疫原

    A synthesized peptide derived from human SF3B3, corresponding to a region within C-terminal amino acids.

    亚型

    IgG
  • 应用备注

    WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20 °C. Stable for 12 months from date of receipt.

    有效期

    12 months
  • 抗原

    SF3B3 (Splicing Factor 3b, Subunit 3, 130kDa (SF3B3))

    别名

    SF3B3

    背景

    Description: Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome (PubMed:10490618, PubMed:10882114, PubMed:27720643, PubMed:28781166). SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA (PubMed:12234937). May also be involved in the assembly of the 'E' complex (PubMed:10882114). Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron (PubMed:15146077).

    Gene: SF3B3

    分子量

    136 kDa

    基因ID

    23450

    UniProt

    Q15393
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