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STAT1 抗体 (pSer727)

Name: STAT1 抗体 (pSer727)
SKU: ABIN6256410
Availability: OutOfStock

This anti-STAT1 antibody is a 兔多克隆 antibody detecting STAT1 in WB, IHC, ELISA, IF 和 ICC. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 1 publication.

Western blot analysis of STAT1 phosphorylation expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (STAT1 抗体 (pSer727))

ABIN6267508 at 1/200 staining Human bladder cancer tissue sections by IHC-P. (STAT1 抗体 (pSer727))

IHC

ABIN6267508 at 1/200 staining Rat lung tissue sections by IHC-P. (STAT1 抗体 (pSer727))

IHC

ABIN6267508 at 1/200 staining Mouse testis tissue sections by IHC-P. (STAT1 抗体 (pSer727))

8 images

(1 reference)

产品编号 ABIN6256410

发货至: 中国

Datasheet as PDF

Quick Overview for STAT1 抗体 (pSer727) (ABIN6256410)

抗原

See all STAT1 抗体

STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This STAT1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

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pSer727

特异性

Phospho-STAT1 (Ser727) Antibody detects endogenous levels of STAT1 only when phosphorylated at Serine 727

交叉反应

人, 小鼠, 大鼠

纯化方法

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

免疫原

A synthesized peptide derived from human STAT1 around the phosphorylation site of Serine 727

亚型

IgG
anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 343-602) antibody
STAT1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (pSer727) antibody
STAT1 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) antibody
STAT1 适用: 人, 犬, 猴 WB, IHC, IHC (p), IF 宿主: 小鼠 Monoclonal 4H9 unconjugated

anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 717-745), (C-Term) antibody
STAT1 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB41406 unconjugated

anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 1-712) antibody
STAT1 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 513-712) antibody
STAT1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 650-750) antibody
STAT1 适用: 人 WB, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 1-712) antibody
STAT1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 1-712) antibody
STAT1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated

anti-Signal Transducer and Activator of Transcription 1, 91kDa (STAT1) (AA 613-712) antibody
STAT1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1A8 unconjugated

应用备注

WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C.Stable for 12 months from date of receipt

有效期

12 months
Wu, Chen, Jing, Gu, Mei, Yao, Zhou, Yang, Sun, Wang, Hu, Wüthrich, Mei: "The C-terminal tail of polycystin-1 regulates complement factor B expression by signal transducer and activator of transcription 1." in: American journal of physiology. Renal physiology, Vol. 310, Issue 11, pp. F1284-94, (2017) (PubMed).
抗原

STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

别名

STAT1

背景

Description: Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
Gene: STAT1

分子量

91kDa

基因ID

6772

UniProt

P42224

途径

JAK/STAT Signaling, RTK signaling, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events