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PERK 抗体 (pThr982)

Name: PERK 抗体 (pThr982)
SKU: ABIN6255756
Availability: OutOfStock

This anti-PERK antibody is a 兔多克隆 antibody detecting PERK in WB, ELISA, IHC, IF 和 ICC. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 3+ publications.

MCF-7 and MDA-MB-231 cells were treated with the indicated concentrations of BA for 24 h, and the protein levels of ER stress-associated signals were stimulated by BA in a dose-dependent manner, including GRP78, p-PERK/PERK, p-eIF2α/eIF2α, CHOP, and caspase-12. (PERK 抗体 (pThr982))

ABIN6277783 at 1/100 staining human liver carcinoma tissue sections by IHC-P. (PERK 抗体 (pThr982))

IHC

IF/ICC

ABIN6277783 staining A549 cells by ICC/IF. (PERK 抗体 (pThr982))

IF/ICC

Western blot analysis of p-PERK (Thr 982) using 293 whole cell lysates (PERK 抗体 (pThr982))

5 images

(3 references)

产品编号 ABIN6255756

发货至: 中国

Datasheet as PDF

Quick Overview for PERK 抗体 (pThr982) (ABIN6255756)

抗原

See all PERK (EIF2AK3) 抗体

PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This PERK antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Discover our top product EIF2AK3 Primary Antibody

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pThr982

特异性

Phospho-PERK (Thr982) Antibody detects endogenous levels of PERK only when phosphorylated at Thr982.

预测反应

Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

纯化方法

Immunogen affinity purified

免疫原

A synthesized peptide derived from human PERK around the phosphorylation site of Thr982.

亚型

IgG
anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 973-1114) antibody
EIF2AK3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (pThr980) antibody
EIF2AK3 适用: 人, 小鼠, 大鼠, 犬, Pig WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (C-Term) antibody
EIF2AK3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 1001-1116) antibody
EIF2AK3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 115-330) antibody
EIF2AK3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 665-764) antibody
EIF2AK3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (N-Term) antibody
EIF2AK3 适用: 人 WB, IHC, IF, IP, IC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (pThr981) antibody
EIF2AK3 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 665-764) antibody
EIF2AK3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 7B10 unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) antibody
EIF2AK3 适用: 人 ELISA 宿主: 小鼠 Monoclonal 5G5 unconjugated

应用备注

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-500, ELISA(peptide) 1:20000-1:40000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store at -20 °C. Stable for 12 months from date of receipt.

有效期

12 months
Cai, Zheng, Gu, Wang, Wang, Yang, Zhang, Wang, Fu, Wang: "Betulinic acid chemosensitizes breast cancer by triggering ER stress-mediated apoptosis by directly targeting GRP78." in: Cell death & disease, Vol. 9, Issue 6, pp. 636, (2018) (PubMed).

Liu, Shen, Wang, Liu, Zheng, Luo: "EndophilinA2 protects against angiotensin II-induced cardiac hypertrophy by inhibiting angiotensin II type 1 receptor trafficking in neonatal rat cardiomyocytes." in: Journal of cellular biochemistry, (2018) (PubMed).

Yao, Lu, Hu, Yu, Chen, Wang: "A non-canonical pathway regulates ER stress signaling and blocks ER stress-induced apoptosis and heart failure." in: Nature communications, Vol. 8, Issue 1, pp. 133, (2017) (PubMed).
抗原

PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

别名

EIF2AK3

背景

Description: Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF-2-alpha/EIF2S1) on 'Ser-52' during the unfolded protein response (UPR) and in response to low amino acid availability. Converts phosphorylated eIF-2-alpha/EIF2S1 either in a global protein synthesis inhibitor, leading to a reduced overall utilization of amino acids, or to a translation initiation activator of specific mRNAs, such as the transcriptional activator ATF4, and hence allowing ATF4-mediated reprogramming of amino acid biosynthetic gene expression to alleviate nutrient depletion. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
Gene: EIF2AK3

分子量

125 kDa

基因ID

9451

UniProt

Q9NZJ5

途径

Hormone Transport, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response