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PERK 抗体 (AA 1001-1116)

Name: PERK 抗体 (AA 1001-1116)
SKU: ABIN740715
Availability: OutOfStock

EIF2AK3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded mouse stomach labeled with Anti-PERK Polyclonal Antibody, Unconjugated (ABIN740715) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PERK Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

U-87MG cells were fixed with 4% PFA for 10min at room temperature, permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature.

3 images

(2 references)

产品编号 ABIN740715

发货至: 中国

Datasheet as PDF

抗原 See all PERK (EIF2AK3) 抗体

PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))
抗原表位
16

15

11

9

7

7

7

6

5

5

4

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 1001-1116
适用
119

74

72

16

16
人, 小鼠, 大鼠
宿主
111

5

3

1
兔
Compatible Secondaries
克隆类型
114

6
多克隆
标记
43

12

9

6

6

5

5

5

5

5

5

4

2

2

2

2

2
This PERK antibody is un-conjugated
应用范围
80

57

45

27

25

25

13

10

8

6

5

3

2

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

人, 小鼠, 大鼠

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human PER

亚型

IgG

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EIF2AK3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (pThr980) antibody
EIF2AK3 适用: 人, 小鼠, 大鼠, 犬, Pig WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (pThr981) antibody
EIF2AK3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (C-Term) antibody
EIF2AK3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) antibody
EIF2AK3 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 115-330) antibody
EIF2AK3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 665-764) antibody
EIF2AK3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (N-Term) antibody
EIF2AK3 适用: 人 WB, IHC, IF, IP, IC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) antibody
EIF2AK3 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Yu, Zhang, Zhao, Guo, Li, Li, Zhang, Sun: "Gypenoside Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting Cardiomyocytes Apoptosis via Inhibition of CHOP Pathway and Activation of PI3K/Akt Pathway In Vivo and In Vitro." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 39, Issue 1, pp. 123-36, (2016) (PubMed).

He, Long, Zhong, Fu, Li, Lin: "Sustained endoplasmic reticulum stress inhibits hepatocyte proliferation via downregulation of c-Met expression." in: Molecular and cellular biochemistry, Vol. 389, Issue 1-2, pp. 151-8, (2014) (PubMed).
抗原

PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

别名

PERK (EIF2AK3 产品)

别名

EIF2AK3 antibody, AI427929 antibody, Pek antibody, Perk antibody, PEK antibody, PERK antibody, WRS antibody, eukaryotic translation initiation factor 2 alpha kinase 3 antibody, EIF2AK3 antibody, Eif2ak3 antibody

背景

Synonyms: PEK, WRS, PERK, Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, HsPEK, EIF2AK3
Background: Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function (By similarity).

基因ID

9451

UniProt

Q9NZJ5

途径

Hormone Transport, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response