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PERK 抗体 (pThr980)

Name: PERK 抗体 (pThr980)
SKU: ABIN744713
Availability: OutOfStock

EIF2AK3 适用: 人, 小鼠, 大鼠, 犬, Pig WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Formalin-fixed and paraffin embedded rat brain labeled with Anti-Phospho-PERK(Thr980) Polyclonal Antibody, Unconjugated (ABIN744713) at 1:200 followed by conjugation to the secondary antibody and DAB staining

IHC

Formalin-fixed and paraffin-embedded human lung carcinoma labeled with Anti-Phospho-PERK(Thr980) Polyclonal Antibody, Unconjugated (ABIN744713) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C.

Paraformaldehyde-fixed, paraffin embedded rat pancreas tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-PERK (Thr980) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining

IHC (p)

3 images

(14 references)

产品编号 ABIN744713

发货至: 中国

Datasheet as PDF

抗原 See all PERK (EIF2AK3) 抗体

PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))
抗原表位
16

15

11

9

7

7

7

6

5

5

4

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
pThr980

适用

人, 小鼠, 大鼠, 犬, Pig
宿主
111

5

3

1
兔
Compatible Secondaries
克隆类型
114

6
多克隆
标记
43

12

9

6

6

5

5

5

5

5

5

4

2

2

2

2

2
This PERK antibody is un-conjugated
应用范围
80

57

45

27

25

25

13

10

8

6

5

3

2

1
Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

交叉反应

犬, 人, 小鼠, Pig, 大鼠

预测反应

Cow,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic phosphopeptide derived from mouse PERK around the phosphorylation site of Thr980

亚型

IgG

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EIF2AK3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (pThr982) antibody
EIF2AK3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (pThr981) antibody
EIF2AK3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (C-Term) antibody
EIF2AK3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 1001-1116) antibody
EIF2AK3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) antibody
EIF2AK3 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 115-330) antibody
EIF2AK3 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (AA 665-764) antibody
EIF2AK3 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) (N-Term) antibody
EIF2AK3 适用: 人 WB, IHC, IF, IP, IC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3) antibody
EIF2AK3 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Kao, Lai, Chow, Liao, Zheng, Tsai, Hsia, Lai, Lee, Chuang, Whang-Peng, Yao: "Opposite Regulation of CHOP and GRP78 and Synergistic Apoptosis Induction by Selenium Yeast and Fish Oil via AMPK Activation in Lung Adenocarcinoma Cells." in: Nutrients, Vol. 10, Issue 10, (2019) (PubMed).

Ju, Su, Chen, Ma, Ji, Wang, Li, Li: "Protective effects of Astragaloside IV on endoplasmic reticulum stress-induced renal tubular epithelial cells apoptosis in type 2 diabetic nephropathy rats." in: Biomedicine & pharmacotherapy, Vol. 109, pp. 84-92, (2019) (PubMed).

Gerbino, Bottillo, Milano, Lipari, Zio, Morlino, Mola, Procino, Re, Zachara, Grammatico, Svelto, Carmosino: "Functional Characterization of a Novel Truncating Mutation in Lamin A/C Gene in a Family with a Severe Cardiomyopathy with Conduction Defects." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 44, Issue 4, pp. 1559-1577, (2018) (PubMed).

Fang, Liu, Wu, Liu, Pan, Li: "Upregulation of long noncoding RNA CCAT1-L promotes epithelial-mesenchymal transition in gastric adenocarcinoma." in: OncoTargets and therapy, Vol. 11, pp. 5647-5655, (2018) (PubMed).

Fang, Yan, Teng, Wen, Li, Peng, Liu, Donadeu, Zhao, Hua: "Melatonin prevents senescence of canine adipose-derived mesenchymal stem cells through activating NRF2 and inhibiting ER stress." in: Aging, Vol. 10, Issue 10, pp. 2954-2972, (2018) (PubMed).

Mak, Yu, Sun, He, Liu, Yang: "Tetramethylpyrazine suppresses angiotensin II-induced soluble epoxide hydrolase expression in coronary endothelium via anti-ER stress mechanism." in: Toxicology and applied pharmacology, Vol. 336, pp. 84-93, (2017) (PubMed).

Chen, Holstein, Aime, Bollo, Lechleiter: "Calcineurin β protects brain after injury by activating the unfolded protein response." in: Neurobiology of disease, Vol. 94, pp. 139-56, (2016) (PubMed).

Carmosino, Gerbino, Schena, Procino, Miglionico, Forleo, Favale, Svelto: "The expression of Lamin A mutant R321X leads to endoplasmic reticulum stress with aberrant Ca(2+) handling." in: Journal of cellular and molecular medicine, Vol. 20, Issue 11, pp. 2194-2207, (2016) (PubMed).

Yu, Zhang, Zhao, Guo, Li, Li, Zhang, Sun: "Gypenoside Protects against Myocardial Ischemia-Reperfusion Injury by Inhibiting Cardiomyocytes Apoptosis via Inhibition of CHOP Pathway and Activation of PI3K/Akt Pathway In Vivo and In Vitro." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 39, Issue 1, pp. 123-36, (2016) (PubMed).

Santanam, Banach-Petrosky, Abate-Shen, Shen, White, DiPaola: "Atg7 cooperates with Pten loss to drive prostate cancer tumor growth." in: Genes & development, Vol. 30, Issue 4, pp. 399-407, (2016) (PubMed).

Xu, Su, Song, Shi, Fu, Hu, Xia, Song, Song: "Polychlorinated biphenyl quinone induces endoplasmic reticulum stress, unfolded protein response, and calcium release." in: Chemical research in toxicology, Vol. 28, Issue 6, pp. 1326-37, (2015) (PubMed).

He, Long, Zhong, Fu, Li, Lin: "Sustained endoplasmic reticulum stress inhibits hepatocyte proliferation via downregulation of c-Met expression." in: Molecular and cellular biochemistry, Vol. 389, Issue 1-2, pp. 151-8, (2014) (PubMed).

Kucuksayan, Konuk, Demir, Mutus, Aslan: "Neutral sphingomyelinase inhibition decreases ER stress-mediated apoptosis and inducible nitric oxide synthase in retinal pigment epithelial cells." in: Free radical biology & medicine, Vol. 72, pp. 113-23, (2014) (PubMed).

Aslan, Basaranlar, Unal, Ciftcioglu, Derin, Mutus: "Inhibition of neutral sphingomyelinase decreases elevated levels of inducible nitric oxide synthase and apoptotic cell death in ocular hypertensive rats." in: Toxicology and applied pharmacology, (2014) (PubMed).
抗原

PERK (EIF2AK3) (Eukaryotic Translation Initiation Factor 2-alpha Kinase 3 (EIF2AK3))

别名

PERK (EIF2AK3 产品)

别名

EIF2AK3 antibody, AI427929 antibody, Pek antibody, Perk antibody, PEK antibody, PERK antibody, WRS antibody, eukaryotic translation initiation factor 2 alpha kinase 3 antibody, EIF2AK3 antibody, Eif2ak3 antibody

背景

Synonyms: Pek, Perk, AI427929, Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase, Eif2ak3
Background: Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.

基因ID

13666

UniProt

Q9Z2B5

途径

Hormone Transport, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response