Nibrin 抗体 (pSer278)
Quick Overview for Nibrin 抗体 (pSer278) (ABIN6255155)
抗原
See all Nibrin (NBN) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer278
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特异性
- Phospho-p95/NBS1 (Ser278) Antibody detects endogenous levels of p95/NBS1 only when phosphorylated at Sersine 278.
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纯化方法
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
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免疫原
- A synthesized peptide derived from human p95/NBS1 around the phosphorylation site of Ser278.
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亚型
- IgG
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anti-Nibrin (NBN) (AA 467-615) antibody
NBN 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 7E4C2 unconjugated
anti-Nibrin (NBN) (AA 183-460) antibodyNBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 8E7 unconjugated
anti-Nibrin (NBN) (AA 461-754) antibodyNBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3A9 unconjugated
anti-Nibrin (NBN) (AA 461-754) antibodyNBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3A2 unconjugated
anti-Nibrin (NBN) (AA 183-460) antibodyNBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 6E11 unconjugated
anti-Nibrin (NBN) (AA 183-460) antibodyNBN 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2F5 unconjugated
anti-Nibrin (NBN) (AA 435-754) antibodyNBN 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nibrin (NBN) (AA 645-754) antibodyNBN 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3E4 unconjugated
anti-Nibrin (NBN) antibodyKO Validated NBN 适用: 人 WB, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Nibrin (NBN) (AA 714-745), (C-Term) antibodyNBN 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
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有效期
- 12 months
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- Nibrin (NBN)
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别名
- NBN
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背景
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Description: Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
Gene: NBN
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分子量
- 85kDa
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基因ID
- 4683
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UniProt
- O60934
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途径
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
抗原
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