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CEA 抗体

This anti-CEA antibody is a 小鼠 单克隆 antibody detecting CEA in WB, FACS, IHC (p) 和 IF. Suitable for 人 和 猴.
产品编号 ABIN6252842
发货至: 中国

Quick Overview for CEA 抗体 (ABIN6252842)

抗原

See all CEA 抗体
CEA (Carcinoembryonic Antigen Gene Family (CEA))

适用

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人, 猴

宿主

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小鼠

克隆类型

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单克隆

标记

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This CEA antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

克隆位点

C66-195
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Recombinant full-length human protein was used as the immunogen for the CEA antibody.

    亚型

    IgG1 kappa
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 1-2 μg/mL

    Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1)

    Optimal dilution of the CEA antibody to be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    CEA (Carcinoembryonic Antigen Gene Family (CEA))

    别名

    CEA

    背景

    This antibody recognizes proteins of 80-200  kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This mAb reacts with nonspecific cross-reacting antigen (NCA) and shows a cross-reaction with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

    基因ID

    1048

    UniProt

    P06731
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