CDC20 抗体
Quick Overview for CDC20 抗体 (ABIN6252686)
抗原
See all CDC20 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G purified antibody
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免疫原
- Denatured recombinant human protein was used as the immunogen for this Cdc20 antibody.
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亚型
- IgG1 kappa
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anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 1-270) antibody
CDC20 适用: 人 WB, IF, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) antibodyCDC20 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 1-270) antibodyCDC20 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 57-84) antibodyCDC20 适用: 人 WB, FACS, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) antibodyCDC20 适用: 人 FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal AR12 unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (N-Term) antibodyCDC20 适用: 人, 小鼠, 大鼠, Pig, 马, 豚鼠, 猴, Cow, 非洲爪蟾, Bat, Hamster WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 1-499) antibodyCDC20 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (N-Term) antibodyCDC20 适用: 人, 小鼠, 大鼠 WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (AA 57-84), (N-Term) antibodyCDC20 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB23122 unconjugated
anti-Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20) (N-Term) antibodyCDC20 适用: 人, 小鼠, 大鼠, Pig, 犬, 马, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cdc20 antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))
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别名
- Cdc20
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背景
- Cyclins, regulatory subunits which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 kinase (p34Cdc2) exhibits kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
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基因ID
- 991
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UniProt
- Q12834
抗原
-
