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GJB1 抗体

This anti-GJB1 antibody is a 大鼠 单克隆 antibody detecting GJB1 in WB, FACS, IF 和 IHC (fro). Suitable for 大鼠 和 小鼠.
产品编号 ABIN6252465
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for GJB1 抗体 (ABIN6252465)

抗原

See all GJB1 抗体
GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

适用

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大鼠, 小鼠

宿主

  • 65
  • 25
  • 11
大鼠

克隆类型

  • 54
  • 47
单克隆

标记

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This GJB1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

R5-21C
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Mouse liver DOC-JR-plasma membranes were used as the immunogen for the Connexin 32 antibody.

    亚型

    IgG2a
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 1-2 μg/mL

    WB: 0.5-1 μg/mL

    IHC (Frozen): 1-2 μg/mL for 30 min at RT

    Optimal dilution of the Connexin 32 antibody should be determined by the researcher.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

    别名

    Connexin 32

    背景

    This Ab recognizes a protein of 27-32  kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.

    基因ID

    14618

    UniProt

    P28230

    途径

    Cell-Cell Junction Organization
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