电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

GJB1 抗体 (Cytoplasmic Loop)

This anti-GJB1 antibody is a 小鼠 单克隆 antibody detecting GJB1 in WB, IF, FACS 和 IHC (fro). Suitable for 人, 大鼠 和 小鼠.
产品编号 ABIN6252464
发货至: 中国

Quick Overview for GJB1 抗体 (Cytoplasmic Loop) (ABIN6252464)

抗原

See all GJB1 抗体
GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

适用

  • 73
  • 72
  • 55
  • 6
  • 4
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
人, 大鼠, 小鼠

宿主

  • 55
  • 25
  • 12
小鼠

克隆类型

  • 54
  • 38
单克隆

标记

  • 45
  • 5
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This GJB1 antibody is un-conjugated

应用范围

  • 53
  • 37
  • 26
  • 25
  • 22
  • 19
  • 13
  • 13
  • 9
  • 6
  • 3
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

M12-13
  • 抗原表位

    • 15
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cytoplasmic Loop

    纯化方法

    Protein G affinity chromatography

    免疫原

    Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).

    亚型

    IgG
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 1-2 μg/mL

    WB: 0.5-1 μg/mL

    IHC (Frozen): 0.5-1 μg/mL for 30 min at RT

    Optimal dilution of the Connexin 32 antibody should be determined by the researcher.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

    别名

    Connexin 32

    背景

    This Ab recognizes a protein of 27-32  kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.

    基因ID

    2705

    UniProt

    P08034

    途径

    Cell-Cell Junction Organization
You are here:
Chat with us!