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CD3 epsilon 抗体 (Cytoplasmic Domain)

CD3E 适用: 人, 小鼠, Pig, 大鼠, 兔, Cow, 马, 豚鼠 FACS, WB, IF, IHC (p) 宿主: 小鼠 Monoclonal PC3-188A unconjugated
产品编号 ABIN6252415
发货至: 中国
  • 抗原 See all CD3 epsilon (CD3E) 抗体
    CD3 epsilon (CD3E)
    抗原表位
    • 24
    • 20
    • 16
    • 16
    • 16
    • 15
    • 12
    • 9
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    • 6
    • 4
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    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 156-168, Cytoplasmic Domain
    适用
    • 217
    • 161
    • 43
    • 41
    • 18
    • 7
    • 7
    • 7
    • 6
    • 4
    • 4
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    • 1
    • 1
    人, 小鼠, Pig, 大鼠, 兔, Cow, 马, 豚鼠
    宿主
    • 143
    • 117
    • 33
    • 22
    • 20
    • 8
    • 1
    小鼠
    克隆类型
    • 217
    • 121
    • 6
    单克隆
    标记
    • 126
    • 31
    • 27
    • 20
    • 15
    • 8
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    • 6
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    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
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    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD3 epsilon antibody is un-conjugated
    应用范围
    • 234
    • 157
    • 86
    • 81
    • 71
    • 70
    • 27
    • 27
    • 24
    • 22
    • 14
    • 14
    • 13
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    • 8
    • 6
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Protein G affinity chromatography
    免疫原
    Amino acids 156-168 (cytoplasmic domain) from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
    克隆位点
    PC3-188A
    亚型
    IgG1
    Top Product
    Discover our top product CD3E Primary Antibody
  • 应用备注
    WB: 1-2 μg/mL

    IHC (FFPE): 1-2 μg/mL for 30 min at RT

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 1-2 μg/mL

    Optimal dilution of the CD3e antibody should be determined by the researcher.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原
    CD3 epsilon (CD3E)
    别名
    CD3e (CD3E 产品)
    别名
    cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody
    背景
    Recognizes the epsilon chain of CD3, a protein which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28  kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
    基因ID
    916
    UniProt
    P07766
    途径
    TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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