CLIP 抗体
Quick Overview for CLIP 抗体 (ABIN6252311)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant full-length human protein was used as the immunogen for the CLIP antibody.
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亚型
- IgG1 kappa
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anti-Corticotropin-Like Intermediate Peptide (CLIP) (AA 156-176) antibody
CLIP 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Corticotropin-Like Intermediate Peptide (CLIP) (AA 156-176) antibodyCLIP 适用: 人 WB 宿主: 小鼠 Monoclonal ABM52H4 unconjugated
anti-Corticotropin-Like Intermediate Peptide (CLIP) (AA 25-39) antibodyCLIP 适用: 人, 大鼠, 小鼠 IHC (p) 宿主: 兔 Monoclonal CLIP-2040R unconjugated
anti-Corticotropin-Like Intermediate Peptide (CLIP) (AA 25-39) antibodyCLIP 适用: 人, 大鼠, 小鼠 IHC (p) 宿主: 兔 Monoclonal CLIP-2040R unconjugated
anti-Corticotropin-Like Intermediate Peptide (CLIP) (AA 25-39) antibodyCLIP 适用: 人 FACS, IF, IHC (p), ELISA 宿主: 小鼠 Monoclonal ADCT1-1 unconjugated
anti-Corticotropin-Like Intermediate Peptide (CLIP) (AA 25-39) antibodyCLIP 适用: 人 FACS, IF, IHC (p), ELISA 宿主: 小鼠 Monoclonal ADCT1-1 unconjugated
anti-Corticotropin-Like Intermediate Peptide (CLIP) antibodyCLIP 适用: 人 FACS, IF, WB 宿主: 小鼠 Monoclonal CLIP-1133 unconjugated
anti-Corticotropin-Like Intermediate Peptide (CLIP) antibodyCLIP 适用: 人 FACS, IF, WB 宿主: 小鼠 Monoclonal CLIP-1133 unconjugated
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应用备注
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Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry: 0.5-1 μg/mL (1)
Prediluted format : incubate for 30 min at RT (2)
Optimal dilution of the CLIP antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- CLIP (Corticotropin-Like Intermediate Peptide (CLIP))
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别名
- CLIP
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背景
- Recognizes proteins of 33, 35 and 41 kDa, which are identified as various isoforms of CD74/CLIP. Its epitope is localized in the extracellular domain of CD74. CD74/CLIP is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. The molecule plays a critical role in the presentation of peptides, by the MHC class II antigens, to CD4 positive lymphocytes. CD74/CLIP is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, and dendritic cells and by various types of carcinomas. It is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74/CLIP stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas and has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
抗原
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