TGFA 抗体 (C-Term)
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Quick Overview for TGFA 抗体 (C-Term) (ABIN6252275)
抗原
See all TGFA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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纯化方法
- Protein G affinity chromatography
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免疫原
- A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF alpha was used as the immunogen for this antibody.
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亚型
- IgG1 kappa
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anti-Transforming Growth Factor, alpha (TGFA) (AA 40-89) antibody
TGFA 适用: 人, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 24-98) antibodyTGFA 适用: 人 IHC, WB, IP, ICC 宿主: 小鼠 Monoclonal D20 unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 24-98) antibodyTGFA 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 40-89) antibodyTGFA 适用: 人 IHC, WB, IF, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) antibodyTGFA 适用: 人, 大鼠, 小鼠 IHC, WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) antibodyTGFA 适用: 人, 大鼠, 小鼠 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 118-150) antibodyTGFA 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB56968 unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (AA 1-160) antibodyTGFA 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Transforming Growth Factor, alpha (TGFA) (C-Term) antibodyTGFA 适用: 人, 大鼠, 小鼠, Cow IHC, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal MF9 unconjugated
anti-Transforming Growth Factor, alpha (TGFA) antibodyTGFA 适用: 人 IHC, WB, ELISA, IHC (p), Neut 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 1-2 μg/mL for 30 min at RT (1)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TGF alpha antibody to be titered up or down for optimal performance.1. Staining of FFPE tissues requires boiling sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- TGFA (Transforming Growth Factor, alpha (TGFA))
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别名
- TGF alpha
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背景
- TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.
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基因ID
- 7039
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UniProt
- P01135
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途径
- NF-kappaB Signaling, RTK signaling, EGFR Signaling Pathway
抗原
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