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CD31 抗体

PECAM1 适用: 人 FACS, WB, IHC, IF 宿主: 小鼠 Monoclonal PCM25-1 unconjugated
产品编号 ABIN6252198
发货至: 中国
  • 抗原 See all CD31 (PECAM1) 抗体
    CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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    小鼠
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    单克隆
    标记
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    This CD31 antibody is un-conjugated
    应用范围
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    Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
    纯化方法
    Protein G affinity chromatography
    免疫原
    Recombinant human protein was used as the immunogen for this CD31 antibody.
    克隆位点
    PCM25-1
    亚型
    IgG1 kappa
    Top Product
    Discover our top product PECAM1 Primary Antibody
  • 应用备注
    Western blot: 1-2 μg/mL

    Flow Cytometry: 0.5-1 μg/10e6 cells

    IF: 1-2 μg/mL

    IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

    Prediluted format : incubate for 30 min at RT (2)

    Titering of the CD31 antibody may be required for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 1  mM EDTA,  pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.2 mg/mL
    缓冲液
    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    -20 °C
    储存方法
    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原
    CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
    别名
    CD31 (PECAM1 产品)
    背景
    CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]
    基因ID
    5175
    UniProt
    P16284
    途径
    Regulation of Actin Filament Polymerization
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