Actin 抗体
Quick Overview for Actin 抗体 (ABIN6252180)
抗原
See all Actin (ACTA1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- SDS extract of human myocardium was used as the immunogen for this Muscle actin HHF35 antibody.
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亚型
- IgG1 kappa
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anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 277-308), (C-Term) antibody
ACTA1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 人, 大鼠, 斑马鱼, 兔, Pig, 小鸡, 猴 WB, ELISA, IHC, IHC (p), IHC (fro), IEM 宿主: 小鼠 Monoclonal HHF35 unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 346-375) antibodyACTA1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 3-377) antibodyACTA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Monoclonal 25E3 unconjugated Recombinant Antibody
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 1-129) antibodyACTA1 适用: 人, 斑马鱼, 鱼 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 346-375), (C-Term) antibodyACTA1 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB35391 unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 人 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal 7C1 unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 7-377) antibodyACTA1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 小鸡 WB, IHC, IF 宿主: 小鼠 Monoclonal C4 unconjugated
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应用备注
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Western blot: 0.5-1 μg/mL
Flow Cytometry: 0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)
Prediluted format : incubate for 30 min at RT (2)
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Muscle actin HHF35 antibody to be titered up or down for optimal performance.1. No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS with 0.1 mg/mL BSA and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot and Store at -20C. Avoid freez-thaw cycles.
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- Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))
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别名
- Muscle Actin
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背景
- This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. This mAb recognizes alpha and gamma isotypes of all muscle groups. Nonmuscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of nonmuscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
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基因ID
- 58
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UniProt
- P68133
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途径
- Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
抗原
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