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Cyclin A 抗体

This anti-Cyclin A antibody is a 小鼠 单克隆 antibody detecting Cyclin A in WB, IF 和 FACS. Suitable for 人, 小鼠, Cow, Green Monkey 和 Mink.
产品编号 ABIN6251854
发货至: 中国
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Quick Overview for Cyclin A 抗体 (ABIN6251854)

抗原

See all Cyclin A (CCNA2) 抗体
Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

适用

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人, 小鼠, Cow, Green Monkey, Mink

宿主

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小鼠

克隆类型

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单克隆

标记

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This Cyclin A antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

E67
  • 纯化方法

    Protein G affinity chromatography

    免疫原

    Full length bovine protein was used as the immunogen for this CCNA2 antibody.

    亚型

    IgG2a kappa
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells

    IF: 1-2 μg/mL





    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin A2 antibody to be titered up or down for optimal performance.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    Cyclin A (CCNA2) (Cyclin A2 (CCNA2))

    别名

    Cyclin A2

    背景

    This antibody recognizes a protein of 50-55 kDa, which is identified as Cyclin A. Its epitope is located amino acids 144-148 of the human protein. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk's) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk's. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, Cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while Cyclin B is a critical regulator of mitosis. Mutation or disruption of normal Cyclin A expression causes cells to arrest in G2-phase.

    基因ID

    890

    UniProt

    P20248

    途径

    PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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