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POMC 抗体 (N-Term)

This 小鼠 单克隆 anti-POMC antibody (Clone 57) specifically detects POMC in WB, IHC, FACS 和 IF. The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN6251838
发货至: 中国
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Quick Overview for POMC 抗体 (N-Term) (ABIN6251838)

抗原

See all POMC 抗体
POMC (Proopiomelanocortin (POMC))

适用

  • 73
  • 48
  • 47
  • 2
  • 1
  • 1
人, 小鼠, 大鼠

宿主

  • 62
  • 32
  • 1
小鼠

克隆类型

  • 52
  • 43
单克隆

标记

  • 58
  • 6
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This POMC antibody is un-conjugated

应用范围

  • 38
  • 35
  • 31
  • 28
  • 18
  • 17
  • 13
  • 13
  • 13
  • 11
  • 9
  • 8
  • 5
  • 5
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

克隆位点

57
  • 抗原表位

    • 16
    • 9
    • 9
    • 6
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    N-Term

    纯化方法

    Protein G affinity chromatography

    免疫原

    An N-terminal peptide from the ACTH region of human POMC was used as the immunogen for this POMC antibody.

    亚型

    IgG1
  • 应用备注

    Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

    Immunofluorescence: 0.5-1 μg/mL

    Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

    The optimal dilution of the ACTH antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.2 mg/mL

    缓冲液

    PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Aliquot and Store at -20C. Avoid freez-thaw cycles.
  • 抗原

    POMC (Proopiomelanocortin (POMC))

    别名

    POMC

    背景

    POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin.

    基因ID

    5443

    UniProt

    P01189

    途径

    Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity, Feeding Behaviour
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