Recombinant Tyrosinase-Related Protein 1 抗体 (Biotin)
Quick Overview for Recombinant Tyrosinase-Related Protein 1 抗体 (Biotin) (ABIN6191389)
抗原
See all Tyrosinase-Related Protein 1 (TYRP1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Recombinant Rabbit Monoclonal anti-Tyrosinase Related Protein-1 (TYRP1/1564R), Biotin Conjugate
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产品特性
- This antibody reacts with a 75 kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi. Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human full-length TYRP1 protein
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亚型
- IgG kappa
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应用备注
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Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- Immunohistology (formalin) 5-10 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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SK-MEL-23, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Tyrosinase-Related Protein 1 (TYRP1)
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别名
- Tyrosinase Related Protein-1
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分子量
- 75 kDa
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基因ID
- 7306, 270279
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UniProt
- P17643
抗原
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