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Melanoma gp100 抗体 (CF®640R)

This 小鼠 单克隆 antibody specifically detects Melanoma gp100 in IF, FACS 和 IHC (f). It exhibits reactivity toward 人 和 马.
产品编号 ABIN6190034
发货至: 中国
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Room 801-803
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Quick Overview for Melanoma gp100 抗体 (CF®640R) (ABIN6190034)

抗原

See all Melanoma gp100 (PMEL) 抗体
Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

适用

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人, 马

宿主

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小鼠

克隆类型

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单克隆

标记

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This Melanoma gp100 antibody is conjugated to CF®640R

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

NKI-beteb
  • 原理

    Mouse Monoclonal anti-Pmel17 / gp100 / SILV (NKI-beteb), CF640R Conjugate

    产品特性

    By immunohistochemistry this antibody specifically recognizes a in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Membranes from a human melanoma metastasis

    亚型

    IgG2b kappa
  • 应用备注

    Immunohistology formalin-fixed 1-2 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Does not react with rat, others not tested
    • Optimal dilution for a specific application should be determined by user

    说明

    SK-MEL-28 cells or Melanoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

    别名

    Pmel17 / gp100 / SILV

    分子量

    90-100 kDa

    基因ID

    6490, 95972

    UniProt

    P40967
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