Melanoma gp100 抗体 (CF®640R)
Quick Overview for Melanoma gp100 抗体 (CF®640R) (ABIN6190034)
抗原
See all Melanoma gp100 (PMEL) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Pmel17 / gp100 / SILV (NKI-beteb), CF640R Conjugate
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产品特性
- By immunohistochemistry this antibody specifically recognizes a in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Membranes from a human melanoma metastasis
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亚型
- IgG2b kappa
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应用备注
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Immunohistology formalin-fixed 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Does not react with rat, others not tested
- Optimal dilution for a specific application should be determined by user
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说明
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SK-MEL-28 cells or Melanoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
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别名
- Pmel17 / gp100 / SILV
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分子量
- 90-100 kDa
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基因ID
- 6490, 95972
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UniProt
- P40967
抗原
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