Melanoma gp100 抗体 (CF®594)
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- 抗原 See all Melanoma gp100 (PMEL) 抗体
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Melanoma gp100 antibody is conjugated to CF®594
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应用范围
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- Mouse Monoclonal anti-Pmel17 / gp100 / SILV (HMB45), CF594 Conjugate
- 产品特性
- By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. This MAb stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This MAb also stains Angiomyolipoma (PEComa). Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 免疫原
- Extract of pigmented melanoma metastases from lymph nodes
- 克隆位点
- HMB45
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product PMEL Primary Antibody
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anti-Premelanosome Protein (PMEL) antibody
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anti-Premelanosome Protein (PMEL) (AA 376-502) antibodyPMEL 适用: 人 IHC, ELISA, StM, Coat 宿主: 小鼠 Monoclonal PMEL-2037 unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal HMB45 unconjugated
anti-Premelanosome Protein (PMEL) (C-Term) antibodyVerified PMEL 适用: 人, 小鼠 IF, WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) (N-Term) antibodyPMEL 适用: 人, 马, 大鼠, 小鼠, 犬, 豚鼠, 兔, Cow, 山羊 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) (AA 25-467) antibodyPMEL 适用: 人 IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人 IHC, StM 宿主: 兔 Monoclonal PMEL-1825R unconjugated Recombinant Antibody
anti-Premelanosome Protein (PMEL) (C-Term) antibodyPMEL 适用: 人, 小鼠 IHC, IF, WB, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Premelanosome Protein (PMEL) antibodyPMEL 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-617R unconjugated Recombinant Antibody
anti-Premelanosome Protein (PMEL) (AA 1-661) antibodyPMEL 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistology (formalin)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Does not react with dog or rat, others not tested
- Optimal dilution for a specific application should be determined by user
- 说明
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SK-MEL-28 cells or Melanoma
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 100 μg/mL
- 缓冲液
- PBS/0.1 % BSA/0.05 % azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Protect from light
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- 抗原
- Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
- 别名
- Pmel17 / gp100 / SILV (PMEL 产品)
- 别名
- D12S53E antibody, ME20 antibody, ME20-M antibody, ME20M antibody, P1 antibody, P100 antibody, PMEL17 antibody, SI antibody, SIL antibody, SILV antibody, gp100 antibody, D10H12S53E antibody, D12S53Eh antibody, Pmel17 antibody, Si antibody, Silv antibody, gp87 antibody, RPE1 antibody, MMP115 antibody, silverb antibody, silver antibody, cb397 antibody, fdv antibody, pmel17 antibody, sb:cb397 antibody, silva antibody, wu:fc11g11 antibody, wu:fj24g11 antibody, zgc:136622 antibody, premelanosome protein antibody, premelanosome protein b antibody, premelanosome protein a antibody, PMEL antibody, Pmel antibody, pmelb antibody, pmela antibody
- 分子量
- 90-100 kDa
- 基因ID
- 6490, 95972
- UniProt
- P40967
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