CD27 抗体 (Biotin)
Quick Overview for CD27 抗体 (Biotin) (ABIN6187741)
抗原
See all CD27 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-CD27 (LPFS2/1611), Biotin Conjugate
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产品特性
- This antibody recognizes a protein of a disulfide-linked 120 kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NF?B and SAPK/JNK and induces apoptosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human CD22 protein (exact sequence is proprietary)
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亚型
- IgG1
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anti-CD27 Molecule (CD27) (AA 1-260) antibody
CD27 适用: 人 WB, ELISA, RNAi 宿主: 小鼠 Monoclonal 1E2-A3 unconjugated
anti-CD27 Molecule (CD27) antibodyCD27 适用: 人 FACS, ELISA, IHC, IF, StM, Coat 宿主: 小鼠 Monoclonal LPFS2-1611 unconjugated
anti-CD27 Molecule (CD27) antibodyCD27 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) (AA 20-191) antibodyCD27 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) (AA 20-144) antibodyCD27 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 3B6D3 unconjugated
anti-CD27 Molecule (CD27) (AA 196-225) antibodyCD27 适用: 人 FACS, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) (AA 229-312) antibodyCD27 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-CD27 Molecule (CD27) antibodyCD27 适用: 人 FACS, IHC, StM 宿主: 小鼠 Monoclonal rLPFS2-1611 unconjugated Recombinant Antibody
anti-CD27 Molecule (CD27) antibodyCD27 适用: 人 FACS 宿主: 小鼠 Monoclonal LT27 unconjugated
anti-CD27 Molecule (CD27) (AA 20-191) antibodyCD27 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM57 unconjugated Recombinant Antibody
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应用备注
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Immunohistology (formalin) 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
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说明
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Human peripheral blood lymphocytes. Human tonsil and lymph node.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- CD27 (CD27 Molecule (CD27))
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别名
- CD27
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背景
- LPFS2, S152, T cell activation antigen S152, T-cell activation antigen CD27, T14, TNFRSF7, TNFSF7, Tp55, Tumor necrosis factor receptor superfamily member 7
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分子量
- 120 kDa
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基因ID
- 939, 355307
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UniProt
- P26842
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途径
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
抗原
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