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CD27 抗体 (CF®594)

This anti-CD27 antibody is a 小鼠 单克隆 antibody detecting CD27 in FACS, IHC 和 IF. Suitable for 人.
产品编号 ABIN6187736
发货至: 中国

Quick Overview for CD27 抗体 (CF®594) (ABIN6187736)

抗原

See all CD27 抗体
CD27 (CD27 Molecule (CD27))

适用

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小鼠

克隆类型

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单克隆

标记

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This CD27 antibody is conjugated to CF®594

应用范围

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

克隆位点

LPFS2-1611
  • 原理

    Mouse Monoclonal anti-CD27 (LPFS2/1611), CF594 Conjugate

    产品特性

    This antibody recognizes a protein of a disulfide-linked 120 kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NF?B and SAPK/JNK and induces apoptosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human CD22 protein (exact sequence is proprietary)

    亚型

    IgG1
  • 应用备注

    Immunohistology (formalin) 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    说明

    Human peripheral blood lymphocytes. Human tonsil and lymph node.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    CD27 (CD27 Molecule (CD27))

    别名

    CD27

    背景

    LPFS2, S152, T cell activation antigen S152, T-cell activation antigen CD27, T14, TNFRSF7, TNFSF7, Tp55, Tumor necrosis factor receptor superfamily member 7

    分子量

    120 kDa

    基因ID

    939, 355307

    UniProt

    P26842

    途径

    Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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