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SUMO1 抗体 (CF®640R)

This anti-SUMO1 antibody is a 小鼠 单克隆 antibody detecting SUMO1 in WB, IF, FACS 和 IHC (f). Suitable for 人 和 大鼠.
产品编号 ABIN6186842
发货至: 中国

Quick Overview for SUMO1 抗体 (CF®640R) (ABIN6186842)

抗原

See all SUMO1 抗体
SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

适用

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人, 大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This SUMO1 antibody is conjugated to CF®640R

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

SM1-495
  • 原理

    Mouse Monoclonal anti-SUMO 1 (SM1/495), CF640R Conjugate

    产品特性

    This MAb is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human SUMO1 protein

    亚型

    IgG1 kappa
  • 应用备注

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Western blotting 0.5-1 μg/mL
    • Predicted to show broad species reactivity
    • Optimal dilution for a specific application should be determined by user

    说明

    Breast carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

    别名

    SUMO 1

    分子量

    11.5 kDa (Monomer), 90 kDa (Heteromer)

    基因ID

    7341, 596171

    UniProt

    P63165

    途径

    M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway
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