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MUC1 抗体 (CF®647)

This anti-MUC1 antibody is a 小鼠 单克隆 antibody detecting MUC1 in FACS, IF 和 IHC (f). Suitable for 人.
产品编号 ABIN6184148
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
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Quick Overview for MUC1 抗体 (CF®647) (ABIN6184148)

抗原

See all MUC1 抗体
MUC1 (Mucin 1 (MUC1))

适用

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小鼠

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单克隆

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This MUC1 antibody is conjugated to CF®647

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

E29
  • 原理

    Mouse Monoclonal anti-Mucin 1 / EMA / Episialin / CD227 (E29), CF647 Conjugate

    产品特性

    In Western blotting, this antibody recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Delipidated extract of human milk fat globule membranes

    亚型

    IgG2a kappa
  • 应用备注

    Immunohistology formalin-fixed 0.1-0.2 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Optimal dilution for a specific application should be determined by user

    说明

    MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    MUC1 (Mucin 1 (MUC1))

    别名

    Mucin 1 / EMA / Episialin / CD227

    分子量

    265-400 kDa

    基因ID

    4582, 89603

    UniProt

    P15941

    途径

    Negative Regulation of intrinsic apoptotic Signaling
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