MUC1 抗体 (CF®405S)
Quick Overview for MUC1 抗体 (CF®405S) (ABIN6184140)
抗原
See all MUC1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Mouse Monoclonal anti-Mucin 1 / EMA / Episialin / CD227 (E29), CF405S Conjugate
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产品特性
- In Western blotting, this antibody recognizes proteins in MW range of 265-400 kDa, identified as different glycoforms of EMA. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Delipidated extract of human milk fat globule membranes
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亚型
- IgG2a kappa
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应用备注
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Immunohistology formalin-fixed 0.1-0.2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
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说明
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MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- MUC1 (Mucin 1 (MUC1))
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别名
- Mucin 1 / EMA / Episialin / CD227
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分子量
- 265-400 kDa
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基因ID
- 4582, 89603
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UniProt
- P15941
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途径
- Negative Regulation of intrinsic apoptotic Signaling
抗原
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