Recombinant Nkx2-2 抗体 (CF®488A)
Quick Overview for Recombinant Nkx2-2 抗体 (CF®488A) (ABIN6181578)
抗原
See all Nkx2-2 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Recombinant Rabbit Monoclonal anti-NKX2.2 (NX2/1422R), CF488A Conjugate
-
产品特性
- Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
免疫原
- Recombinant human NKX2.2 protein fragment (exact sequence is proprietary)
-
亚型
- IgG kappa
-
-
-
-
应用备注
-
Immunohistology (formalin) 1-2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
-
说明
-
Pancreas or Ewing s sarcoma
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 100 μg/mL
-
缓冲液
- PBS/0.1 % BSA/0.05 % azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Protect from light
-
-
- Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))
-
别名
- NKX2.2
-
背景
- Homeobox protein NK-2 homolog B, NK2 transcription factor like protein B, NK2 transcription factor related locus 2, NKX22, Nkx2b, tinman
-
分子量
- 40-50 kDa
-
基因ID
- 4821, 516922
-
UniProt
- O95096
-
途径
- Dopaminergic Neurogenesis
抗原
-