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Recombinant Nkx2-2 抗体

This 小鼠 单克隆 anti-Nkx2-2 antibody (Clone rNX2-294) specifically detects Nkx2-2 in IHC, IF 和 FACS. The antibody is reactive with 人, 小鼠, 大鼠 和 小鸡 samples.
产品编号 ABIN6181555
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Recombinant Nkx2-2 抗体 (ABIN6181555)

抗原

See all Nkx2-2 抗体
Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

抗体类型

Recombinant Antibody

适用

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人, 小鼠, 大鼠, 小鸡

宿主

  • 85
  • 57
小鼠

克隆类型

  • 92
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单克隆

标记

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This Nkx2-2 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

克隆位点

RNX2-294
  • 原理

    Recombinant Mouse Monoclonal anti-NKX2.2 (rNX2/294), Purified, BSA-free

    产品特性

    Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93 % and a specificity of 89 %, and aids in the differential diagnosis of small round cell tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    纯化方法

    Purified

    免疫原

    Human full-length recombinant NKX2.2 protein

    亚型

    IgG2b kappa
  • 应用备注

    Immunohistology (formalin) 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Optimal dilution for a specific application should be determined by user

    说明

    Pancreas or Ewing s sarcoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS (no BSA, no azide)

    储存液

    Azide free
  • 抗原

    Nkx2-2 (NK2 Homeobox 2 (Nkx2-2))

    别名

    NKX2.2

    背景

    Homeobox protein NK-2 homolog B, NK2 transcription factor like protein B, NK2 transcription factor related locus 2, NKX22, Nkx2b, tinman

    分子量

    40-50 kDa

    基因ID

    4821, 516922

    UniProt

    O95096

    途径

    Dopaminergic Neurogenesis
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