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PODXL 抗体 (Extracellular Domain) (CF®594)

This anti-PODXL antibody is a 小鼠 单克隆 antibody detecting PODXL in WB, IP, FACS, IF 和 IHC (f). Suitable for 人, 大鼠 和 兔.
产品编号 ABIN6180288
发货至: 中国

Quick Overview for PODXL 抗体 (Extracellular Domain) (CF®594) (ABIN6180288)

抗原

See all PODXL 抗体
PODXL (Podocalyxin-Like (PODXL))

适用

  • 106
  • 27
  • 14
  • 11
  • 1
  • 1
人, 大鼠, 兔

宿主

  • 68
  • 55
  • 1
小鼠

克隆类型

  • 70
  • 54
单克隆

标记

  • 67
  • 10
  • 7
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
This PODXL antibody is conjugated to CF®594

应用范围

  • 74
  • 66
  • 33
  • 33
  • 32
  • 24
  • 21
  • 18
  • 12
  • 8
  • 5
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

克隆位点

3D3
  • 抗原表位

    • 23
    • 16
    • 9
    • 8
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain

    原理

    Mouse Monoclonal anti-Podocalyxin (3D3), CF594 Conjugate

    产品特性

    Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

    亚型

    IgG1
  • 应用备注

    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Immunoprecipitation 1-2 μg/500 μg protein
    • Optimal dilution for a specific application should be determined by user

    说明

    HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    100 μg/mL

    缓冲液

    PBS/0.1 % BSA/0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light
  • 抗原

    PODXL (Podocalyxin-Like (PODXL))

    别名

    Podocalyxin

    背景

    PODXL, PDXL, GCTM-2, Gp200, PCLP1, Pcx, Podocalyxin-like protein 1

    分子量

    165-170 kDa

    基因ID

    5420, 732423

    UniProt

    O00592

    途径

    Tube Formation
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