PODXL 抗体 (Extracellular Domain) (CF®488A)
Quick Overview for PODXL 抗体 (Extracellular Domain) (CF®488A) (ABIN6180286)
抗原
See all PODXL 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Extracellular Domain
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原理
- Mouse Monoclonal anti-Podocalyxin (3D3), CF488A Conjugate
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产品特性
- Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin
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亚型
- IgG1
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应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Immunoprecipitation 1-2 μg/500 μg protein
- Optimal dilution for a specific application should be determined by user
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说明
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HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- PODXL (Podocalyxin-Like (PODXL))
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别名
- Podocalyxin
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背景
- PODXL, PDXL, GCTM-2, Gp200, PCLP1, Pcx, Podocalyxin-like protein 1
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分子量
- 165-170 kDa
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基因ID
- 5420, 732423
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UniProt
- O00592
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途径
- Tube Formation
抗原
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