GRP94 抗体 (CF®594)
Quick Overview for GRP94 抗体 (CF®594) (ABIN6179642)
抗原
See all GRP94 (HSP90B1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Rat Monoclonal anti-Glucose Regulated Protein 94 (GRP94) (9G10.F8.2), CF594 Conjugate
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产品特性
- This antibody recognizes a protein of 94 kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Purified glucose regulated protein 94 (grp94) from chicken oviducts
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亚型
- IgG2a kappa
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anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 550-650) antibody
HSP90B1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (Center) antibodyHSP90B1 适用: 人, 小鼠 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibodyHSP90B1 适用: 人, 小鼠, 大鼠 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (Internal Region) antibodyVerified HSP90B1 适用: 人, 小鼠 WB, IHC, FACS, IF, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 676-803) antibodyHSP90B1 适用: 人 WB, IF, IHC (p), EIA 宿主: 小鼠 Monoclonal 2H3 unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibodyHSP90B1 适用: 人, 小鼠, 大鼠, Cow, 小鸡, Hamster, 犬, 兔, Pig, 豚鼠, 猴, 绵羊, 马, 非洲爪蟾 WB, IHC, FACS, IF, StM 宿主: 大鼠 Monoclonal 9G10-F8-2 unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibodyHSP90B1 适用: 人, 小鼠, 大鼠, Cow, 小鸡, Hamster, 犬, 兔, Pig, 豚鼠, 猴, 绵羊, 马, 非洲爪蟾 IHC, FACS, StM 宿主: 兔 Monoclonal HSP90B1-3168R unconjugated Recombinant Antibody
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibodyHSP90B1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 687-700), (Middle Region) antibodyHSP90B1 适用: 人, 大鼠 WB, IHC, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 676-803) antibodyHSP90B1 适用: 人 WB, FACS, IF, ELISA, ICC 宿主: 小鼠 Monoclonal 2H3 unconjugated
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应用备注
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Immunohistology (Formalin-fixed) 0.5-1.0 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 0.5-1.0 μg/mL
- Western blotting 0.5-1.0 μg/mL
- Optimal dilution for a specific application should be determined by user
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说明
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U20S cells. About 50% of breast tumors express grp94 protein
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 100 μg/mL
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缓冲液
- PBS/0.1 % BSA/0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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- GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
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别名
- Glucose Regulated Protein 94
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分子量
- 94 kDa
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基因ID
- 7184, 192374
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UniProt
- P14625
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途径
- Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades
抗原
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